This popular 2-week laboratory-based training course provides a comprehensive overview and practical laboratory experience of the genetic manipulation of mammalian stem cells, focusing on human iPS cells.

The intensive, hands-on programme covers cutting-edge techniques in this exciting and fast-moving field, including genome informatics, vector construction employing recombineering and synthetic biology methods, and genome editing strategies using CRISPR-Cas9. The course also includes interactive demonstrations, discussions and lectures by international experts.

Laboratory work includes the use of CRISPR-Cas9 for advanced genome engineering and genetic screens, the design and construction of gene targeting vectors, the culture and transfection of human iPS cells and the derivation of iPS cells and organoid stem cells. An emphasis will be placed on the planning/design and successful experimental execution of gene targeting projects and participants should gain the skills necessary to design, construct and target their own genes of interest.

The course is aimed at postdoctoral scientists or senior PhD students actively engaged in, or soon to commence, relevant research. The course is also suitable for facility managers/principal investigators wishing to bring the latest genetic modification technologies into their facilities/groups.


Feedback from the 2016 course

  • "The course was excellent, very well-run and planned and highly useful to my current and planned projects. I appreciated the access and engagement with instructors, speakers and coursemates, which provided multiple avenues for learning and discussion. I enjoyed the intellectual experience, and appreciate the time and efforts of the instructors, course organisers and administrative and laboratory staff."
  • "This is one of the best courses I have visited so far."
  • "Overall the course was of a very high standard with all the instructors doing a great job and their time and effort is really appreciated."
  • "Overall the quality and standard of the course was excellent. The team of instructors/organizers was absolutely fantastic and the panel of speakers is great."
  • "I very much enjoyed the course and have learned a lot. I would like to thank the organisers for putting in all this effort to organise a fantastic course."
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(or call us: +44 (0)1223 496910)


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