Run jointly with EMBL-EBI, this course provides hands-on training in the basics of mass spectrometry and proteomics bioinformatics, search engines and post-processing software, quantitative approaches, MS data repositories, the use of public databases for protein analysis, annotation of subsequent protein lists and incorporation of information from molecular interaction and pathway databases.

The course is aimed at research scientists with a minimum of a degree in a biological discipline, including laboratory and clinical staff, as well as specialists in related fields. The practical elements of the course will take raw data from a proteomics experiment and analyse it. Participants will be able to go from MS spectra, to identifying peptides and finally to lists of protein identifiers that can be analysed further using a wide range of resources. The final aim is to provide attendees with the practical bioinformatics knowledge they need to go back to the lab and process their own data (including large datasets) when collected.

Learning Outcomes
After completing the course, participants should be able to:

  • Use and understand bioinformatics tools to analyse shotgun proteomics data, involving identification and quantification approaches
  • Browse, search, submit and retrieve proteomics data from widely used public repositories
  • Discuss standards in proteomics bioinformatics and recognise its importance
  • Evaluate the strengths and weaknesses of several experimental and bioinformatics analysis approaches
  • Use tools to perform functional annotation of lists of proteins

Feedback from the 2016 course

"I really enjoyed the course. I feel that I learned a lot, and I feel confident with practising on most of the tools were talked about"
"Best course I have been on. Heavy workload but lectures/tutorials were mostly delivered at the right speed with a few exceptions. Very well organised and wonderful surroundings."
"Overall, this course was great! Each of the topics were good and the speakers were phenomenal! The instructors were great! Well organized!"
"I found the course very valuable and well organised, and will utilise much of the information provided here. I am looking forward to analysing my own data with the tools provided."
"I would highly recommend this course to anyone that wants to use proteomics and bioinformatics in the future! I'm glad that I was able to participate!"
"Excellent course and useful for anyone carrying out proteomics bioinformatics. Also suitable for both biologists and bioinformaticians".

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