Across all biological sciences, technological advances result in more ambitious projects and larger data sets. This course will demonstrate how to take common immunological techniques to the next level by combining them with semi-automated and automated analysis methods and using appropriate statistical analysis pipelines.

This practical, laboratory-based course is suited to PhD students and postdocs who would like to get more out of the experiments they perform and make sure their data analysis has a sound basis.

Participants will receive training in how to set up an immunological phenotyping experiment and will gain hands-on experience in a number of immunological techniques suitable for large-scale phenotyping. Using the data generated during the course, we will explore manual, semi-automated and automated analysis approaches and relevant statistics for different data sets and discuss benefits and challenges. All experiments will be conducted on samples from a knockout strain which the participants will identify at the end of the course.

The course will be conducted in collaboration with the Wellcome Trust Sanger Institute Mouse Genetics Project (MGP) and the Infection and Immunity Immunophenotyping (3i) consortium.

MGP generates and phenotypes knockout mice, most of which were generated by the International Knockout Mouse Consortium. For each mutant line, groups of seven male and seven female mice move through a standard analysis pipeline aimed at detecting traits that differ from healthy C57BL/6N mice. The pipeline collects many measurements of viability, fertility, body weight, infection, hearing, morphology, haematology, behaviour, blood chemistry and immunity and compares them to wild type controls. An overview over the first 250 lines has been published in Cell by White and al. Currently, MGP phenotypes 160 knockout lines a year.

During the course we will delve hands-on into a number of tests that are conducted routinely by 3i under high-throughput conditions and discuss the best way to analyse and interpret the data.

Feedback from the 2017 course

"I am grateful for the opportunity to learn at the WGC. I will definitely recommend this course for anyone interested in both clinical and translational immunology."
"I can honestly say that this has been by far the best course I have attended. It was both stimulating and relevant."
"Great experience. Thanks!"
"I felt the course was fantastic. The hard work of everybody involved was evident and highly appreciated."
"I am very grateful for the opportunity to attend this course. It was very relevant, interesting, and complex information
was made accessible to the audience, despite heterogeneous background knowledge. Highly recommended."
"Please send my greatest regard to everyone involved in the organization. They did a fantastic job. Specifically, many many thanks to Nicola, Simon, Lucie, Adam, Anneliese, Kate and Julie! They were all extremely helpful!"
"Just to say again how fantastic the course was. I would like to say a huge thank you to all of the organisers and supervisors. I feel extremely lucky and privileged to have been able to attend. I would very strongly recommend the course to anyone, and have already been telling people how great it was!"
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