This free-to-attend, laboratory-based course will provide participants with a working knowledge of, and practical experience in, cutting-edge Plasmodium experimental genetics techniques; from designing gene targeting vectors and creating transgenic parasites to genotyping and phenotyping the resulting strains.

The 2018 course will be held in Bangkok, Thalland in collaboration with Mahidol University Faculty of Tropical Medicine and is open to applicants from Asia and Australasia.

The goal is to facilitate the participants’ own research careers by exposing them to state-of-the-art experimental approaches, while discussing the advantages and limitations of each approach. The course is targeted to early career malaria scientists from Asia and Australasia who are looking to establish and use Plasmodium transfection technology at their host institutes.

The week-long course will cover experimental approaches to generating genetic mutations in the human malaria parasites Plasmodium falciparum and Plasmodium knowlesi The course is hands-on and attendees will generate gene targeting constructs, perform different transfection approaches, undertake practical exercises in genotyping of modified parasites, and both observe and learn advanced molecular phenotyping approaches.

There will be a particular focus on new scalable approaches, including CRISPR-Cas9 genome editing. Experimental genetic approaches in other malaria parasites including the rodent malaria model Plasmodium berghei will also be discussed. The laboratory-based programme will be complemented by data analysis sessions and specific lectures in cutting-edge areas presented by distinguished guest speakers.

The overall programme strategy will be to follow a Plasmodium experimental genetics experiment from conception to completion, equipping attendees to design and carry out their own projects in the future.

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