This course type is laboratory

Chromatin Structure and Function

29 October - 08 November 2017Wellcome Genome Campus, Hinxton, UK
Deadlines (at 23:59 UTC):
  • Application Deadline Closed

  • Summary

    This popular laboratory- and computer-based course provides training in
    experimental and computational approaches that can be used to
    mechanistically dissect important aspects of chromatin biology at the
    gene and genome-wide levels.

    Our understanding of eukaryotic genomes has benefitted tremendously from
    whole genome sequencing projects. However, we are only beginning to
    systematically understand how the interplay of DNA, proteins and its
    biochemical modifications (i.e., chromatin) can influence global
    processes which control gene expression, mRNA splicing, and DNA
    replication or repair in normal development and in disease.

    Learning Outcomes
    After attending the course, participants can expect to be able to:

    • Perform chromatin immunoprecipitation, ATAC, 3C and Capture C, and RRBS techniques, including experimental design, end-point analysis and validation (qPCR; FISH)
    • Understand the methods used in the preparation of material for next generation sequencing (NGS)
    • Perform end-to-end analysis of NGS data, derived from the above methods, from raw sequence through to identifying relevant loci.
    • Visualise large-scale datasets in formats suitable for figures for publication
    • Download and install relevant software and manage large datasets.
    • Understand how polymer physics can be used to build models of chromatin structures
    Feedback from the 2016 course
    “I had never used bioinformatic tools before, and the “step-by-step” introduction we received in the course was, in my opinion, one of the most useful things I learned. Also, I take home nice advice and experience from the ATAC, FISH, RRBS and Capture-C and ChIP.”
    “Everything was very organized and useful. Great group of Instructors!”
    “Thank you all again for this great opportunity.”
    “Thanks you very much for organising such an amazing course!”
    “Thanks so much for organizing this course so meticulously! I appreciated every single module and came out of these nine days with a whole new understanding of how I can approach my thesis project. Very grateful for the opportunity to attend, and hope to see you all at some point in the not-so-distant future!”
    “Thank you to all the instructors. I’m am really really grateful for all the hard work that they put into planning and executing the course. I feel like I gained a years worth of knowledge in just 9 days. I already feel like a more informed scientist.”
    “The course was immensly useful to all the students and all the instructors were perfect mentors to us.”
    “All instructors did an exceptionally good job – all material was very well explained and instructors were approachable and friendly – thanks!”
  • Programme

    Participants will receive intensive wet laboratory and computational
    training focussed across five broad themes using a variety of
    state-of-the-art methods:

    • DNA/transcription factor interactions and histone dynamics
      Methods: Chromatin immunoprecipitation (ChIP)
    • DNA methylation
      Methods: reduced representation bisulfite sequencing (RRBS)
    • Chromatin accessibility
      Methods: Assay for Transposase-Accessible Chromatin (ATAC)
    • Chromatin looping and nuclear organisation
      Methods: Chromosome conformation capture (3C), Capture-C, and fluorescent in situ hybridisation (FISH)
    • Computational analysis/integration of chromatin datasets
      Methods: basic programming in Unix, sequence alignments, data visualisation tools, ChiP-seq analysis, RRBS analysis, 3C analysis

    View the full 2017 draft timetable here.

    These sessions will be supplemented with informal tutorials and lectures
    from the instructors and distinguished invited speakers. Participants
    will analyse various genome-wide datasets to complement the wet
    laboratory sessions. They will also have an opportunity to discuss their
    own research interests and present aspects of their work that are
    relevant to the course.

    Learning Outcomes
    After attending the course, participants can expect to be able to:

    • Perform
      chromatin immunoprecipitation, ATAC, 3C and Capture C, and RRBS
      techniques, including experimental design, end-point analysis and
      validation (qPCR; FISH)
    • Understand the methods used in the preparation of material for next generation sequencing (NGS)
    • Perform end-to-end analysis of NGS data, derived from the above methods, from raw sequence through to identifying relevant loci.
    • Visualise large-scale datasets in formats suitable for figures for publication
    • Download and install relevant software and manage large datasets.
    • Understand how polymer physics can be used to build models of chromatin structures
  • Instructors / Speakers

    Course instructors
    David
    Vetrie
    University of Glasgow, UK
    Nick
    Gilbert
    University of Edinburgh, UK
    Paul Guilhamon Princess Margaret Cancer Centre,
    Canada
    Robert Andrews Cardiff University, UK

    Guest speakers
    Andrew Feber University College London,
    UK
    Nada Jabado McGill University, USA
    Benjamin Rowland Netherlands Cancer Institute, The Netherlands

  • How to Apply

    Prerequisites
    Applicants should be PhD students, post-doctoral scientists or clinicians
    engaged in relevant research.

    Cost

    The
    course is subsidised by the Wellcome Genome Campus
    Advanced Courses and Scientific Conferences Programme. This is a
    residential
    course and there is a fee of £1195 towards
    board and lodging for non-commercial applicants. Please contact us for
    the commercial fee.

    Additional limited bursaries are available (up to 50%
    of the course fee)
    and are awarded on merit. Please see the “Bursaries”
    tab for details.

    Applications
    Applications for this course can be completed online. Places are limited
    and will be awarded on merit. If you have any problems with the online
    application process, please contact us.

    Please note: Applications
    must be supported by a
    recommendation from a scientific or clinical sponsor (e.g. supervisor or
    head of department). A request for a supporting
    statement will be sent
    to your nominated sponsor automatically during
    the application process.
    Applicants must ensure that their sponsor
    provides this supporting
    statement by the application deadline. Applications without a supporting
    statement cannot be considered.

    Deadlines
    Deadline for Applications: Closed

    Travel visas
    Please contact the
    event organiser if you require a letter to support a travel visa
    application. Note that letters will be provided to confirmed attendees.

    Non-European Economic Area or Swiss nationals may be required to have a
    visa to enter the UK.
    Early application is strongly advised, as this process can take 6-8 weeks
    or longer.

    Please visit the following websites for further information:
    UK Border Agency website and information for general visitors and business
    visitors.

  • Cost / Bursaries

    Cost
    The
    course is subsidised by the Wellcome Genome Campus Advanced Courses
    and
    Scientific Conferences Programme. This is a residential
    course and
    there is a fee of £1195 towards board and lodging for
    non-commercial applicants. Please contact us for the commercial fee.

    Bursaries
    Advanced Courses are subsidised for non-commercial
    applicants from anywhere in the world. Additional, limited bursaries are
    available (up to 50% of the course fee) and are awarded on merit. If you
    would like to apply for a
    bursary, please complete the bursary section
    of the online application
    form.

    Please note that both the applicant
    and sponsor are required to provide
    a justification for the
    bursary as part of the application.

    Bursary terms and conditions

    UK Courses (held at the Wellcome Genome Campus, Hinxton,
    Cambridge)
    A
    limited number of bursaries are available for each course. These are
    awarded by the selection committee according to merit. The bursary
    covers a maximum of 50% of the course fee, though in exceptional
    circumstances an application for the total course fee may be considered.
    Where there are many bursary applications, the selection committee may
    issue smaller amounts. We cannot assist with travel costs to attend UK
    courses.

    Overseas Courses (held outside of the UK)
    A
    limited number of bursaries are available for each course. These are
    awarded on merit to cover travel, accommodation and sustenance. The
    maximum award for travel (economy class) will be £750.

    Bursaries can be applied for as part of the course application form.
    Applicants
    will be notified of a bursary award along with their place on
    the
    course, usually within one month of the application deadline. The
    decision of the selection committee is
    final.