This course type is laboratory

Derivation and Culture of Human Induced Pluripotent Stem Cells (hiPSCs)

12 - 15 December 2016Wellcome Genome Campus, Hinxton, UK
Deadlines (at 23:59 UTC):
  • Application Deadline Closed

  • Summary

    Established in 2015 in collaboration with the UK Stem Cell Bank, this
    4-day practical course includes lectures, discussions and
    laboratory-based training sessions covering current theory and best
    practices with regard to derivation and culture of human induced
    pluripotent stem cells (hiPSCs).

    Learning Outcomes
    After attending this course, participants should be able to:

    • Recognise key principles of good cell culture practice (GCCP) and aseptic processing including the role of antibiotics
    • Assess and identify key advantages, disadvantages  and critical elements of different approaches to somatic cell reprogramming
    • Assess and identify undifferentiated, fully reprogrammed iPSCs compared to partially reprogrammed iPSC colonies
    • Recognise critical aspects for successful preservation and storage
    • Evaluate the recovery of iPSCs and identify viable cells
    • Identify undifferentiated morphology of confluent iPSCs ready for passaging
    • Identify and troubleshoot critical aspects in the passaging and maintenance of undifferentiated iPSCs
    • Recognise the importance of core QC and potential consequences of failing to carry it out
    • Evaluate different capabilities of each method of determining potential pluripotency

    Feedback from the inaugural 2015 course:

    “It was a wonderful experience.”
    “Thank you. I had a great time. You guys were awesome and supportive.”

    “Thanks to everyone running the course, had a thoroughly enjoyable time
    and learnt lots.”
    “The scientific seminars were very informative (particularly the CRISPR
    seminar).”
    “Instructors were excellent & really helpful & sociable.”

  • Programme

    The programme will include lecture, discussion and practical laboratory
    sessions covering the following topics:

    • Good Cell Culture Practice (GCCP) and aseptic processing
    • Approaches for reprogramming of somatic cells (reprogramming methodologies – virus and episomal; somatic cell types – skin fibroblasts and PBMCs)
    • Cryopreservation and recovery
    • Passaging
    • Quality control (identity, karyology, mycoplasma, sterility, cell markers)
    • Pluripotency testing

    Learning Outcomes
    After attending this course, participants should be able to:

    • Recognise key principles of GCCP and aseptic processing including the role of antibiotics
    • Assess and identify key advantages, disadvantages  and critical elements of different approaches to somatic cell reprogramming
    • Assess and identify undifferentiated, fully reprogrammed iPSCs compared to partially reprogrammed iPSC colonies
    • Recognise critical aspects for successful preservation and storage
    • Evaluate the recovery of iPSCs and identify viable cells
    • Identify undifferentiated morphology of confluent iPSCs ready for passaging
    • Identify and troubleshoot critical aspects in the passaging and maintenance of undifferentiated iPSCs
    • Recognise the importance of core QC and potential consequences of failing to carry it out
    • Evaluate different capabilities of each method of determining potential pluripotency
  • Instructors / Speakers

    Lead Instructors
    Glyn
    Stacey
    UK Stem Cell Bank, The National Institute for
    Biological Standards and Control (NIBSC), UK
    Minal Patel Wellcome Trust Sanger Institute, UK

    Guest Speakers
    Andrew Bassett Wellcome Trust Sanger Institute, UK
    Laurence Daheron Harvard Stem Cell Institute, USA
    Daniel
    Gaffney
    Wellcome Trust Sanger Institute, UK

  • How to Apply

    Prerequisites
    Applicants should be scientists or clinicians engaged in relevant
    research.

    Cost
    The
    course is subsidised by the Wellcome Genome Campus Advanced Courses
    and Scientific Conferences Programme. This is a residential
    course and
    there is a fee of £435 towards board and lodging for non-commercial
    applicants. Please contact us for the commercial fee.

    Additional limited bursaries are available (up to 50%
    of the course fee)
    and are awarded on merit. Please see the “Bursaries”
    tab for details.

    Applications
    Applications for this course can be completed online. If you have any
    problems with the online application process, please contact us.

    Please note: Applications
    must be supported by a
    recommendation from a scientific or clinical sponsor (e.g. supervisor or
    head of department). A request for a supporting
    statement will be sent
    to your nominated sponsor automatically during
    the application process.
    Applicants must ensure that their sponsor
    provides this supporting
    statement by the application deadline. Applications without a supporting
    statement cannot be considered.

    Deadlines
    Deadline for Applications: Closed

  • Bursaries

    Bursaries
    Advanced Courses are subsidised for non-commercial applicants from
    anywhere in the world. Additional, limited bursaries are
    available (up
    to 50% of the course fee) and are awarded on merit. If you would like to
    apply for a
    bursary, please complete the bursary section of the online
    application
    form.

    Please note that both the applicant
    and sponsor are required to provide
    a justification for the
    bursary as part of the application.

    Bursary terms and conditions

    UK Courses (held at the Wellcome Genome Campus, Hinxton,
    Cambridge)
    A
    limited number of bursaries are available for each course. These are
    awarded by the selection committee according to merit. The bursary
    covers a maximum of 50% of the course fee, though in exceptional
    circumstances an application for the total course fee may be considered.
    Where there are many bursary applications, the selection committee may
    issue smaller amounts. We cannot assist with travel costs to attend UK
    courses.

    Overseas Courses (held outside of the UK)
    A
    limited number of bursaries are available for each course. These are
    awarded on merit to cover travel, accommodation and sustenance. The
    maximum award for travel (economy class) will be £750.

    Bursaries can be applied for as part of the course application form.
    Applicants
    will be notified of a bursary award along with their place on
    the
    course, usually within one month of the application deadline. The
    decision of the selection committee is
    final.