This course type is laboratory

Genetic Engineering of Mammalian Stem Cells

11 - 23 April 2016Wellcome Genome Campus, Hinxton, UK
Deadlines (at 23:59 UTC):
  • Application Deadline Closed

  • Summary

    This laboratory-based training course will provide a comprehensive
    overview and practical laboratory experience of the genetic manipulation
    of mammalian stem cells, focusing on human iPS cells. Strategies for
    genome engineering of mouse models and mutant mouse resources produced by
    the International Mouse Phenotyping Consortium (IMPC) will also be
    discussed. Genome informatics, vector construction employing
    recombineering and synthetic biology methods, and genome editing
    strategies using CRISPR-Cas9 will be covered in interactive
    demonstrations, discussions and lectures by international experts.

    Laboratory work includes the use CRISPR-Cas9 for advanced genome
    engineering and genetic screens, the design and construction of gene
    targeting vectors, the culture and transfection of human iPS cells and
    the derivation of iPS cells and organoid stem cells. An emphasis will be
    placed on the planning/design and successful experimental execution of
    gene targeting projects and participants should gain the skills necessary
    to design, construct and target their own genes of interest.

    Feedback from the 2015 course
    “The quality and attitude of the instructors was world-class; they
    created a top tier course and delivered state-of-the-art information. I
    would highly recommend this Advanced Course to anyone seeking to perform
    genetic manipulations in mammalian cells. My deepest thanks to all those
    involved in putting this together.”
    “This was an amazing course! It covered a wide range of state of the art
    topics and the practical parts were perfect to get in close contact to
    these experts in their fields. The motivation and performance of all
    teachers and lecturers was outstanding and encouraging! I want to thank
    all the organizers, teachers, lecturers and participants for this great
    experience.”
    “This is an awesome course! The instructors are amazing and their
    dedication and commitment in organizing such a course every year is
    commendable. The selection of course participants is also excellent.
    Wonderful all around.”
    “This course is amazing! Thank you to all the wonderful
    instructors!”

  • Programme

    The programme will include lecture and practical
    laboratory/computer-based sessions covering the following topics:

    1. Informatics
    The visualization and interpretation of gene models will be presented
    focusing on the practical design of gene targeting strategies. Students
    will also develop their own CRISPR-mediated gene targeting designs using
    web-based tools and learn how to exploit the public IMPC mouse gene
    targeting/phenotyping resources.

    2. Recombineering of BACs and Gene Targeting Vectors

    Participants will use recombineering to make modified BAC transgenes and
    gene targeting constructs. The theoretical principles underlying both
    recombineering and rational targeting vector design will be presented in
    lectures and practical exercises.

    3. Modular Gene Targeting Vectors
    Students will learn to apply modular approaches to vector assembly and
    gene targeting strategies and engage in designing and building their own
    vectors for CRISPR-assisted gene targeting.

    4. Stem Cell Culture/Genome Editing
    Genome editing experiments will be performed where students will learn
    feeder-free culture of human iPS cells and transfection techniques for
    plasmid- and protein-based delivery of Cas9 to engineer a variety of
    useful allelesPicking, expansion, archiving and genotyping of iPS cell
    clones will also be covered.

    5. Genome-wide screens
    Practical application of the CRISPR system to undertake in vitro genetic
    screens will also be explored. This includes the design of genome-wide
    screens, lentiviral transduction of mouse ES cells and bioinformatic
    analysis of next-generation sequencing data.

    6. iPS Cell Generation
    Students will be introduced to protocols for highly efficient
    reprogramming of mouse and human somatic cells to induced pluripotent
    stem cells.

    7. Organoid derivation and differentiation
    Organoids (primary 3D epithelial culture) can be derived from many
    tissues of any mouse strain and enables us to explore gene functions in
    diverse types of primary cells. A protocol for the establishment of
    murine intestinal organoids will be introduced. Several transgenic
    organoids will be shown as examples

  • Instructors / Speakers

    Course instructors
    Francis Stewart Dresden University of Technology,
    Dresden, Germany
    William Skarnes Wellcome Trust Sanger Institute,
    Cambridge, UK
    Pentao Liu Wellcome Trust Sanger Institute, Cambridge,
    UK
    Barry Rosen AstraZeneca, Cambridge, UK
    Kosuke Yusa Wellcome Trust Sanger Institute, Cambridge,
    UK
    Bon-Kyoung Koo Wellcome Trust – MRC Cambridge Stem Cell
    Institute, Cambridge, UK

    Confirmed guest speakers
    Jacob Corn University of California, Berkeley,
    USA
    David Frendewey
    Regeneron Pharmaceuticals, Inc., USA
    Austin Smith Wellcome Trust Centre for Stem Cell
    Research, Cambridge, UK
    Kevin Eggan Harvard Stem Cell Institute, Massachusetts,
    USA
    Nick Hastie Medical Research Council, UK
    Christine Mummery Leiden University Medical Centre, The
    Netherlands
    Jennifer Nichols Wellcome Trust Centre for Stem Cell
    Research, Cambridge, UK
    Allan Bradley Wellcome Trust Sanger Institute,
    Cambridge, UK
    Gavin Kelsey Babraham Institute, UK
    Martin Jinek
    University of Zurich, Switzerland

  • How to Apply

    Prerequisites
    Applicants should be postdoctoral scientists or senior PhD students
    actively engaged in or soon to commence relevant research. The course is
    also suitable for facility managers/principal investigators wishing to
    bring the latest genetic modification technologies into their
    facilities/groups.

    Cost
    The
    course is subsidised by the Wellcome Genome Campus Advanced Courses
    and Scientific Conferences Programme. This is a residential
    course and
    there is a fee of £2100 towards board and lodging for non-commercial
    applicants. Please contact us for the commercial fee.

    Applications
    Applications for this course can now be completed online. If you have any
    problems with the online application process, please contact us.

    Please note: Applications
    must be supported by a
    recommendation from a scientific or clinical sponsor (e.g. supervisor or
    head of department). A request for a supporting
    statement will be sent
    to your nominated sponsor automatically during
    the application process.
    Applicants must ensure that their sponsor
    provides this supporting
    statement by the application deadline. Applications without a supporting
    statement cannot be considered.

    Deadlines
    Deadline for Applications: Closed

  • Bursaries

    Bursaries
    Advanced Courses are subsidised for non-commercial applicants from
    anywhere in the world. Limited bursaries are
    available (up to 50% of the
    course fee) and are awarded on merit. If you would like to apply for a
    bursary, please complete the bursary section of the online application
    form.

    Please note that both the applicant
    and sponsor are required to provide
    a justification for the
    bursary as part of the application.

    Bursary terms and conditions

    UK Courses (held at the Wellcome Genome Campus, Hinxton,
    Cambridge)
    A
    limited number of bursaries are available for each course. These are
    awarded by the selection committee according to merit. The bursary
    covers a maximum of 50% of the course fee, though in exceptional
    circumstances an application for the total course fee may be considered.
    Where there are many bursary applications, the selection committee may
    issue smaller amounts. We cannot assist with travel costs to attend UK
    courses.

    Overseas Courses (held outside of the UK)
    A
    limited number of bursaries are available for each course. These are
    awarded on merit to cover travel, accommodation and sustenance. The
    maximum award for travel (economy class) will be £750.

    Bursaries can be applied for as part of the course application form.
    Applicants
    will be notified of a bursary award along with their place on
    the
    course, usually within one month of the application deadline. The
    decision of the selection committee is
    final.