This course type is laboratory

Genetic Engineering of Mammalian Stem Cells

12 - 24 March 2017Wellcome Genome Campus, Hinxton, UK
Deadlines (at 23:59 UTC):
  • Deadline for Applications Closed
  • Application Deadline Closed

  • Summary

    This popular 2-week laboratory-based training course provides a
    comprehensive overview and practical laboratory experience of the genetic
    manipulation of mammalian stem cells, focusing on human iPS cells.

    The intensive, hands-on programme covers cutting-edge techniques in this
    exciting and fast-moving field, including genome informatics, vector
    construction employing recombineering and synthetic biology methods, and
    genome editing strategies using CRISPR-Cas9. The course also includes
    interactive demonstrations, discussions and lectures by international
    experts.

    Laboratory work includes the use of CRISPR-Cas9 for advanced genome
    engineering and genetic screens, the design and construction of gene
    targeting vectors, the culture and transfection of human iPS cells and
    the derivation of iPS cells and organoid stem cells. An emphasis will be
    placed on the planning/design and successful experimental execution of
    gene targeting projects and participants should gain the skills necessary
    to design, construct and target their own genes of interest.

    The course is aimed at postdoctoral scientists or senior PhD students
    actively engaged in, or soon to commence, relevant research. The course
    is also suitable for facility managers/principal investigators wishing to
    bring the latest genetic modification technologies into their
    facilities/groups.

    Feedback from the 2016 course

    • “The course was excellent, very well-run and planned and highly useful to my current and planned projects. I appreciated the access and engagement with instructors, speakers and coursemates, which provided multiple avenues for learning and discussion. I enjoyed the intellectual experience, and appreciate the time and efforts of the instructors, course organisers and administrative and laboratory staff.”
    • “This is one of the best courses I have visited so far.”
    • “Overall the course was of a very high standard with all the instructors doing a great job and their time and effort is really appreciated.”
    • “Overall the quality and standard of the course was excellent. The team of instructors/organizers was absolutely fantastic and the panel of speakers is great.”
    • “I very much enjoyed the course and have learned a lot. I would like to thank the organisers for putting in all this effort to organise a fantastic course.”
  • Programme

    The programme will include lecture and practical
    laboratory/computer-based sessions covering the following topics:

    Informatics
    The visualization and interpretation of gene models will be presented
    focusing on the practical design of gene targeting strategies. Students
    will also have the opportunity to develop their own CRISPR-mediated gene
    targeting designs using web-based tools and learn how to exploit public
    genomic data resources.

    Modular Gene Targeting Vectors
    Students will learn to apply modular approaches to vector assembly and
    gene targeting strategies and engage in the construction and analysis of
    vectors for CRISPR-assisted gene targeting.

    Stem Cell Culture/Genome Editing
    Genome editing experiments will be performed where students will learn
    feeder-free culture of human iPS cells and transfection techniques for
    plasmid- and protein-based delivery of Cas9 to engineer a variety of
    useful alleles. Cell manipulation techniques including picking,
    expansion, archiving and genotyping of iPS cell clones will also be
    covered.

    Genome-wide screens
    Practical application of the CRISPR system to undertake in vitro genetic
    screens will also be explored. This includes the design of genome-wide
    screens, lentiviral transduction of mouse ES cells and bioinformatic
    analysis of next-generation sequencing data.

    iPS Cell Generation
    Students will be introduced to protocols for highly efficient
    reprogramming of mouse and human somatic cells to induced pluripotent
    stem cells.

    Organoid derivation and differentiation
    Organoids (primary 3D epithelial culture) can be derived from many
    tissues of any mouse strain and enables us to explore gene functions in
    diverse types of primary cells. A protocol for the establishment of
    murine intestinal organoids will be introduced. Several transgenic
    organoids will be shown as examples

    Learning Outcomes
    After attending this course, participants will be able to:

    • Assess targeting strategies within a genomic context, identifying suitable targets and CRISPR reagents
    • Design and build vectors for CRISPR-assisted gene targeting using modular construction approaches
    • Apply cellular reprogramming methods to derive induced pluripotent stem cells for human and mouse
    • Undertake genome editing of human iPS cells, including manipulation of cells under culture conditions, applying different transfection techniques
    • Develop genome-wide screens exploiting lentiviral transduction methods, including analysis using next-generation sequencing
    • Investigate new research techniques, exploiting tissue-derived organoids to explore gene function and cellular interactions
  • Instructors / Speakers

    Course instructors
    Andrew Bassett
    Wellcome Trust Sanger Institute, UK
    Bon-Kyoung
    Koo
    Wellcome Trust – MRC Cambridge Stem Cell Institute,
    UK
    Pentao
    Liu
    Wellcome Trust Sanger Institute, UK
    Barry Rosen AstraZeneca, Cambridge, UK
    Mark
    Thomas
    Wellcome Trust Sanger Institute, UK
    Kosuke
    Yusa
    Wellcome Trust Sanger Institute, UK

    Guest speakers
    Chad Cowan
    Harvard Stem Cell Institute, USA
    Hans Clevers
    Hubrecht
    Institute, The Netherlands
    Frank
    Buchholz
    University of Dresden, Germany

    David Frendewey Regeneron, USA
    Christine
    Mummery
    Leiden University Medical Center, The Netherlands

    Kathy
    Niakan

    Francis Crick Institute, UK
    Jennifer
    Nichols

    Wellcome Trust Centre for Stem Cell Research, UK
    Wolf
    Reik
    Babraham Institute, UK

  • How to Apply

    Prerequisites
    Applicants should be postdoctoral scientists or
    senior PhD students
    actively engaged in, or soon to commence, relevant
    research. The course
    is also suitable for facility managers/principal
    investigators wishing
    to bring the latest genetic modification
    technologies into their
    facilities/groups.

    Cost
    The
    course is subsidised by the Wellcome Genome Campus
    Advanced Courses and Scientific Conferences Programme. This is a
    residential
    course and there is a fee of £2140 towards
    board and lodging for non-commercial applicants. Please contact us for
    the commercial fee.

    Additional limited bursaries are available (up to 50%
    of the course fee)
    and are awarded on merit. Please see the “Bursaries”
    tab for details.

    Applications
    Applications for this course can be completed online. If you have any
    problems with the online application process, please contact us.

    Please note: Applications
    must be supported by a
    recommendation from a scientific or clinical sponsor (e.g. supervisor or
    head of department). A request for a supporting
    statement will be sent
    to your nominated sponsor automatically during
    the application process.
    Applicants must ensure that their sponsor
    provides this supporting
    statement by the application deadline. Applications without a supporting
    statement cannot be considered.

    Deadlines
    Deadline for Applications: Closed

    Travel visas
    Please contact the
    event organiser if you require a letter to support a
    travel visa
    application. Note that letters will be provided to confirmed
    attendees.

    Non-European Economic Area or Swiss nationals may be required to have a
    visa to enter the UK.
    Early application is strongly advised, as this process can take 6-8 weeks
    or longer.

    Please visit the following websites for further information:
    UK Border Agency website and information for general visitors and business
    visitors.

  • Cost / Bursaries

    Cost
    The
    course is subsidised by the Wellcome Genome Campus
    Advanced Courses and
    Scientific Conferences Programme. This is a
    residential
    course and there is a fee of £2140 towards
    board and lodging for non-commercial applicants. Please contact us for
    the commercial fee.

    Bursaries
    Advanced Courses are subsidised for non-commercial
    applicants from anywhere in the world. Additional, limited bursaries are
    available (up to 50% of the course fee) and are awarded on merit. If you
    would like to apply for a
    bursary, please complete the bursary section
    of the online application
    form.

    Please note that both the applicant
    and sponsor are required to provide
    a justification for the
    bursary as part of the application.

    Bursary terms and conditions

    UK Courses (held at the Wellcome Genome Campus, Hinxton,
    Cambridge)
    A
    limited number of bursaries are available for each course. These are
    awarded by the selection committee according to merit. The bursary
    covers a maximum of 50% of the course fee, though in exceptional
    circumstances an application for the total course fee may be considered.
    Where there are many bursary applications, the selection committee may
    issue smaller amounts. We cannot assist with travel costs to attend UK
    courses.

    Overseas Courses (held outside of the UK)
    A
    limited number of bursaries are available for each course. These are
    awarded on merit to cover travel, accommodation and sustenance. The
    maximum award for travel (economy class) will be £750.

    Bursaries can be applied for as part of the course application form.
    Applicants
    will be notified of a bursary award along with their place on
    the
    course, usually within one month of the application deadline. The
    decision of the selection committee is
    final.