This course type is laboratory

Immunophenotyping: Generation and Analysis of Immunological Datasets

21 - 27 February 2016Wellcome Genome Campus, Hinxton, UK
Deadlines (at 23:59 UTC):
  • Application Deadline Closed

  • Summary

    Across all biological sciences, technological advances result in more ambitious projects and larger datasets. This course will demonstrate how to take common immunological techniques to the next level by combining them with semi-automated and automated analysis methods and using appropriate statistical analysis pipelines. The course is suited for PhD students and postdocs who would like to get more out of the experiments they perform and make sure their data analysis has a sound basis.

    This new, practical, laboratory-based course will be conducted in
    collaboration with the Wellcome
    Trust Sanger Institute Mouse Genetics Project
    and the Infection and
    Immunity Immunophenotyping (3i) consortium

    Participants will receive hands-on training in how to set-up an
    immunological phenotyping experiment and a number of immunological
    techniques suitable for large-scale phenotyping. Using the data generated
    during the course, we will explore manual, semi-automated and automated
    analysis approaches and relevant statistics for different data sets and
    discuss benefits and challenges. All experiments will be conducted on
    samples from a knockout strain which the participants will identify at
    the end of the course.

    After having attended this course, the participants will be able to apply the immunological techniques used during the course in their own research and will be able to develop better experimental, analytical and statistical approaches to basic research projects and high-throughput projects alike.

  • Programme

    The programme will include lecture and practical
    laboratory/computer-based sessions covering the following topics:

    Immunological techniques:

    • Detection of antinuclear antibodies in serum
    • CD8 degranulation assay
    • 12-colour panel design for various immune cell populations
    • Flow-cytometric analysis of blood, spleen, lymph nodes and bone marrow
    • Microscopic analysis of the immune compartment in mouse skin

    Analysis techniques:

    • Analysis of 2D microscopic images with a macro
    • Analysis of 3D microscopy data with a Definiens Developer algorithm
    • Analysis of multidimensional flow data with automated and semi-automated approaches
    • Use of appropriate statistical techniques to analyse the generated datasets
  • Instructors / Speakers

    Course instructors
    Adrian Hayday King’s College London, UK
    Simon Clare Wellcome Trust Sanger Institute, UK
    Lucie Abeler-Dörner King’s College London, UK

    Confirmed guest speakers
    William Jacobs Jr.
    Albert Einstein College of Medicine, USA

    Richard Grencis University of Manchester, UK
    Gillian Griffiths University of Cambridge, UK
    Ryan Brinkman BC Cancer Agency, Canada

  • How to Apply

    The course is aimed at PhD students, postdocs or clinicians conducting
    relevant research.

    course is subsidised by the Wellcome Genome Campus Advanced Courses
    and Scientific Conferences Programme. This is a residential
    course and
    there is a fee of £745 towards board and lodging for non-commercial
    applicants. Please contact us for the commercial fee.

    Applications for this course can now be completed online. If you have any
    problems with the online application process, please contact us.

    Please note: Applications
    must be supported by a
    recommendation from a scientific or clinical sponsor (e.g. supervisor or
    head of department). A request for a supporting
    statement will be sent
    to your nominated sponsor automatically during
    the application process.
    Applicants must ensure that their sponsor
    provides this supporting
    statement by the application deadline. Applications without a supporting
    statement cannot be considered.

    Deadline for Applications: Closed

  • Bursaries

    Advanced Courses are subsidised for non-commercial applicants from
    anywhere in the world. Additional limited bursaries are
    available (up to
    50% of the course fee) and are awarded on merit. If you would like to
    apply for a
    bursary, please complete the bursary section of the online

    Please note that both the applicant
    and sponsor are required to provide
    a justification for the
    bursary as part of the application.

    Bursary terms and conditions

    UK Courses (held at the Wellcome Genome Campus, Hinxton,
    limited number of bursaries are available for each course. These are
    awarded by the selection committee according to merit. The bursary
    covers a maximum of 50% of the course fee, though in exceptional
    circumstances an application for the total course fee may be considered.
    Where there are many bursary applications, the selection committee may
    issue smaller amounts. We cannot assist with travel costs to attend UK

    Overseas Courses (held outside of the UK)
    limited number of bursaries are available for each course. These are
    awarded on merit to cover travel, accommodation and sustenance. The
    maximum award for travel (economy class) will be £750.

    Bursaries can be applied for as part of the course application form.
    will be notified of a bursary award along with their place on
    course, usually within one month of the application deadline. The
    decision of the selection committee is