This course type is laboratory

Immunophenotyping: Generation and Analysis of Immunological Datasets

18 - 24 February 2018Wellcome Genome Campus, Hinxton, UK
Deadlines (at 23:59 UTC):
  • Application Deadline Closed

  • Summary

    Across all biological sciences, technological advances result in more
    ambitious projects and larger data sets. This course will demonstrate how
    to take common immunological techniques to the next level by combining
    them with semi-automated and automated analysis methods and using
    appropriate statistical analysis pipelines.

    This practical, laboratory-based course is suited to PhD students and
    postdocs who would like to get more out of the experiments they perform
    and make sure their data analysis has a sound basis.

    Participants will receive training in how to set up an immunological
    phenotyping experiment and will gain hands-on experience in a number of
    immunological techniques suitable for large-scale phenotyping. Using the
    data generated during the course, we will explore manual, semi-automated
    and automated analysis approaches and relevant statistics for different
    data sets and discuss benefits and challenges. All experiments will be
    conducted on samples from a knockout strain which the participants will
    identify at the end of the course.

    The course will be conducted in collaboration with the Wellcome Trust Sanger Institute Mouse Genetics
    Project
    (MGP) and the Infection and Immunity Immunophenotyping (3i)
    consortium
    .

    MGP generates and phenotypes knockout mice, most of which were generated
    by the International Knockout Mouse Consortium. For each mutant line,
    groups of seven male and seven female mice move through a standard
    analysis pipeline aimed at detecting traits that differ from healthy
    C57BL/6N mice. The pipeline collects many measurements of viability,
    fertility, body weight, infection, hearing, morphology, haematology,
    behaviour, blood chemistry and immunity and compares them to wild type
    controls. An overview over the first 250 lines has been published in Cell by White and al. Currently, MGP
    phenotypes 160 knockout lines a year.

    During the course we will delve hands-on into a number of tests that are
    conducted routinely by 3i under high-throughput conditions and discuss
    the best way to analyse and interpret the data.

    Feedback from the 2017 course

    “I am grateful for the opportunity to learn at the WGC. I will definitely recommend this course for anyone interested in both clinical and translational immunology.”
    “I can honestly say that this has been by far the best course I have attended. It was both stimulating and relevant.”
    “Great experience. Thanks!”
    “I felt the course was fantastic. The hard work of everybody involved was evident and highly appreciated.”
    “I am very grateful for the opportunity to attend this course. It was very relevant, interesting, and complex information
    was made accessible to the audience, despite heterogeneous background knowledge. Highly recommended.”
    “Please send my greatest regard to everyone involved in the organization. They did a fantastic job. Specifically, many many thanks to Nicola, Simon, Lucie, Adam, Anneliese, Kate and Julie! They were all extremely helpful!”
    “Just to say again how fantastic the course was. I would like to say a huge thank you to all of the organisers and supervisors. I feel extremely lucky and privileged to have been able to attend. I would very strongly recommend the course to anyone, and have already been telling people how great it was!”
  • Programme

    The programme will include lecture and practical
    laboratory/computer-based sessions covering the following topics:

    Immunological techniques:

    • Detection of antinuclear antibodies in serum
    • CD8 degranulation assay
    • 12-colour panel design for various immune cell populations
    • Flow-cytometric analysis of blood, spleen, lymph nodes and bone marrow
    • Microscopic analysis of the immune compartment in mouse skin
    Analysis techniques:
    • Analysis of 2D microscopic images with a macro
    • Analysis of 3D microscopy data with ImageJ
    • Analysis of multidimensional flow data with automated and semi-automated approaches
    • Use of appropriate statistical techniques to analyse the generated datasets

    Please note: The course assumes participants have some
    basic experience and knowledge of immunology including fundamentals of
    flow cytometry and FlowJo analysis.

    Learning outcomes
    After having attended this course, participants will be able to

    • measure antinuclear antibodies in serum
    • assess degranulation of cytotoxic cells
    • prepare and stain skin samples for use on a confocal microscope
    • use basic algorithms to analyse 3D confocal data in different software programmes
    • employ a high-throughput approach to large-scale sample preparation of blood, spleen, and bone marrow for flow-cytometric analysis
    • analyse and interpret high-dimensional flow cytometry data
    • use appropriate statistical tests to analyse data generated during the course
    • explain how sources of variation can impact data
    • manage sources of variation through experimental design
    • assess when multiple testing will lead to false positives
    • develop a work plan for analysing mice at their home institution
  • Instructors / Speakers

    Course instructors
    Adrian
    Hayday
    King’s College London, UK
    Simon
    Clare
    Wellcome Trust Sanger Institute, UK
    Lucie
    Abeler-Dörner
    King’s College London, UK
    Anneliese
    Speak
    Wellcome Trust Sanger Institute, UK
    Adam
    Laing
    King’s College London, UK
    Dmitry
    Ushakov
    King’s College London, UK

    Guest speakers
    Ian Humphreys (Cardiff University UK)
    William R. Jacobs, Jr. (Albert Einstein College of Medicine, USA)

  • How to Apply

    Prerequisites
    The course is aimed at PhD students, postdocs or clinicians conducting
    relevant research. Please note: The
    course assumes
    participants will have some basic experience and knowledge of
    immunology
    including fundamentals of flow cytometry and FlowJo analysis.

    Cost
    The
    course is subsidised by the Wellcome Genome Campus Advanced Courses
    and Scientific Conferences Programme. This is a residential
    course and
    there is a fee of £775 towards board and lodging for
    non-commercial applicants. Please contact us for the commercial fee.

    Additional limited bursaries are available (up to 50%
    of the course fee)
    and are awarded on merit. Please see the “Bursaries”
    tab for details.

    Applications
    Applications for this course can be completed online. Places are limited
    and will be awarded on merit. If you have any problems with the online
    application process, please contact us.

    Please note: Applications
    must be supported by a
    recommendation from a scientific or clinical sponsor (e.g. supervisor or
    head of department). A request for a supporting
    statement will be sent
    to your nominated sponsor automatically during
    the application process.
    Applicants must ensure that their sponsor
    provides this supporting
    statement by the application deadline. Applications without a supporting
    statement cannot be considered.

    Deadlines
    Deadline for Applications: Closed

    Travel visas
    Please contact the
    event organiser if you require a letter to support a travel visa
    application. Note that letters will be provided to confirmed attendees.

    Non-European Economic Area or Swiss nationals may be required to have a
    visa to enter the UK.
    Early application is strongly advised, as this process can take 6-8 weeks
    or longer.

    Please visit the following websites for further information:
    UK Border Agency website and information for general visitors and business
    visitors.

  • Cost / Bursaries

    Cost
    The
    course is subsidised by the Wellcome Genome Campus Advanced Courses
    and
    Scientific Conferences Programme. This is a residential
    course and
    there is a fee of £775 towards board and lodging for
    non-commercial applicants. Please contact us for the commercial fee.

    Bursaries
    Advanced Courses are subsidised for non-commercial applicants from
    anywhere in the world. Additional, limited bursaries are
    available (up
    to 50% of the course fee) and are awarded on merit. If you would like to
    apply for a
    bursary, please complete the bursary section of the online
    application
    form.

    Please note that both the applicant
    and sponsor are required to provide
    a justification for the
    bursary as part of the application.

    Bursary terms and conditions

    UK Courses (held at the Wellcome Genome Campus, Hinxton,
    Cambridge)
    A
    limited number of bursaries are available for each course. These are
    awarded by the selection committee according to merit. The bursary
    covers a maximum of 50% of the course fee, though in exceptional
    circumstances an application for the total course fee may be considered.
    Where there are many bursary applications, the selection committee may
    issue smaller amounts. We cannot assist with travel costs to attend UK
    courses.

    Overseas Courses (held outside of the UK)
    A
    limited number of bursaries are available for each course. These are
    awarded on merit to cover travel, accommodation and sustenance. The
    maximum award for travel (economy class) will be £750.

    Bursaries can be applied for as part of the course application form.
    Applicants
    will be notified of a bursary award along with their place on
    the
    course, usually within one month of the application deadline. The
    decision of the selection committee is
    final.