This course type is laboratory

Malaria Experimental Genetics

08 - 14 May 2016Wellcome Genome Campus, Hinxton, UK
Deadlines (at 23:59 UTC):
  • Application Deadline Closed

  • Summary

    This laboratory-based advanced course will give participants a working
    knowledge of and practical experience in cutting edge Plasmodium
    experimental genetics techniques, from designing gene targeting vectors
    and creating transgenic parasites to phenotyping the strains produced.
    The goal is to facilitate the participants’ own research careers by
    exposing them to state-of-the-art experimental approaches, discussing the
    advantages and limitations of each approach, and facilitating networking.

    The week-long residential course will cover experimental approaches to
    generating genetic mutations in both human parasites (Plasmodium
    and Plasmodium knowlesi) and rodent parasites
    (Plasmodium berghei). The course is hands-on and attendees will
    generate gene targeting constructs, carry out different transfection
    approaches, undertake practical exercises in gentoyipng of modified
    parasites, and both observe and learn advanced molecular phenotyping
    approaches. There will be a particular focus on new scalable approaches,
    including CRISPR-Cas9 genome editing and parallel phenotyping by barcode
    sequencing. The laboratory-based programme will be complemented by
    specific lectures in cutting-edge areas presented by a panel of
    distinguished guest speakers.

    Feedback from previous courses
    “This was a great course, very well organized and full of useful
    material, I will definitely recommend to others”
    “It is a great opportunity, unique. I am extremely grateful to Wellcome
    by have been able to participate”
    “I am certain that the knowledge that I gained in this course will aid me
    in progressing my research.”
    “I think the course was absolutely wonderful and I learnt a lot. All the
    organisers were awesome.”

  • Programme

    The overall programme strategy will be to follow a Plasmodium experimental genetics experiment from conception to completion, equipping attendees to design and carry out their own projects in the future. Topics to be covered will include:

    Gene targeting vector construction

    • Recombineering-based approaches to vector construction
    • CRISPR-Cas9 based approaches
    • Consideration of the relative merits of different conditional expression approaches

    Transfection and genotyping

    • Mechanics of transfection in P. berghei, P. knowlesi and P. falciparum
    • Verification of targeting using multiple genotyping techniques

    Molecular phenotyping

    • Imaging approaches
    • Application of next generation sequencing to transcriptional profiling and quantitating parasite growth
    • Flow cytometry-based phenotyping platforms
  • Instructors / Speakers

    Course instructors
    Dr Oliver Billker Wellcome Trust Sanger Institute,
    Hinxton, UK
    Dr Marcus Lee Wellcome Trust Sanger Institute, Hinxton,
    Dr Julian Rayner Wellcome Trust Sanger Institute,
    Hinxton, UK

    Guest speakers
    Dan Goldberg Washington University, USA
    Tania De Koning-Ward
    Deakin University, Australia
    Nisha Philip
    University of Glasgow, UK
    Artur Scherf
    Institut Pasteur, France
    Moritz Treeck
    The Francis Crick Institute, UK

  • How to Apply

    This course is aimed at postdoctoral scientists, PhD students or
    clinician scientists engaged in, or soon to commence, relevant research.

    course is subsidised by the Wellcome Genome Campus Advanced Courses
    and Scientific Conferences Programme. This is a residential
    course and
    there is a fee of £765 towards board and lodging for non-commercial
    applicants. Please contact us for the commercial fee.

    Applications for this course can be completed online. If you have any
    problems with the online application process, please contact us.

    Please note: Applications
    must be supported by a
    recommendation from a scientific or clinical sponsor (e.g. supervisor or
    head of department). A request for a supporting
    statement will be sent
    to your nominated sponsor automatically during
    the application process.
    Applicants must ensure that their sponsor
    provides this supporting
    statement by the application deadline. Applications without a supporting
    statement cannot be considered.

    Deadline for Applications: Closed

  • Bursaries

    Advanced Courses are subsidised for non-commercial applicants from
    anywhere in the world. Limited bursaries are
    available (up to 50% of the
    course fee) and are awarded on merit. If you would like to apply for a
    bursary, please complete the bursary section of the online application

    Please note that both the applicant
    and sponsor are required to provide
    a justification for the
    bursary as part of the application.

    Bursary terms and conditions

    UK Courses (held at the Wellcome Genome Campus, Hinxton,
    limited number of bursaries are available for each course. These are
    awarded by the selection committee according to merit. The bursary
    covers a maximum of 50% of the course fee, though in exceptional
    circumstances an application for the total course fee may be considered.
    Where there are many bursary applications, the selection committee may
    issue smaller amounts. We cannot assist with travel costs to attend UK

    Overseas Courses (held outside of the UK)
    limited number of bursaries are available for each course. These are
    awarded on merit to cover travel, accommodation and sustenance. The
    maximum award for travel (economy class) will be £750.

    Bursaries can be applied for as part of the course application form.
    will be notified of a bursary award along with their place on
    course, usually within one month of the application deadline. The
    decision of the selection committee is