Malaria Experimental Genetics (Accra, Ghana)

30 April - 06 May 2017Accra, Ghana
Deadlines (at 23:59 UTC):
  • Application Deadline Closed

  • Summary

    In collaboration with the University of Ghana, we are pleased to announce
    this Wellcome Genome Campus Overseas Course in Malaria Experimental
    Genetics.

    This free laboratory-based course will provide participants with hands-on
    training in cutting-edge Plasmodium experimental genetics
    techniques, from designing gene targeting vectors and creating transgenic
    parasites to genotyping and phenotyping the resulting strains.

    The goal is to facilitate the participants’ own research careers by
    exposing them to state-of-the-art experimental approaches, while
    discussing the advantages and limitations of each approach.

    The week-long course will cover experimental approaches to generating
    genetic mutations in the human malaria parasite Plasmodium
    falciparum
    . The course is hands-on and attendees will generate gene
    targeting constructs, carry out different transfection approaches,
    undertake practical exercises in genotyping of modified parasites, and
    both observe and learn advanced molecular phenotyping approaches.

    There will be a particular focus on new scalable approaches, including
    CRISPR-Cas9 genome editing. Experimental genetic approaches in other
    malaria parasites including the rodent malaria model Plasmodium
    berghei
    and the human pathogen Plasmodium knowlesi will
    also be discussed. The laboratory-based programme will be complemented by
    specific lectures
    in cutting-edge areas presented by distinguished
    regional guest
    speakers.

    The course is open to scientists and clinicians based in Africa who are
    looking to establish and use Plasmodium transfection technology
    at their institutes. Places are limited and priority will be given to
    applicants who provide a strong scientific case for learning
    Plasmodium genome modification.

    The course will be taught in English.

  • Programme

    The overall programme strategy will be to follow a Plasmodium
    experimental genetics experiment from conception to completion, equipping
    attendees to design and carry out their own projects in the future.
    Topics to be covered will include:

    Gene targeting vector construction

    • CRISPR-Cas9 based approaches
    • Consideration of the relative merits of different conditional expression approaches

    Transfection and genotyping

    • Mechanics of transfection in  P. falciparum
    • Verification of targeting using multiple genotyping techniques

    Molecular phenotyping

    • Imaging approaches
    • Application of next generation sequencing to transcriptional profiling and quantitating parasite growth
    • Flow cytometry-based phenotyping platforms


    Learning Outcomes

    After attending this course, participants will be able to:

    • design CRISPR-Cas9 gene targeting constructs to engineer single point mutations and gene disruptions
    • plan and execute P. falciparum transfection experiments, and genotyping of the resulting transfection progeny
    • understand the strengths and limits of different reverse genetic techniques, including conditional gene technologies
    • appreciate the range of phenotypic assays available for malaria experimental genetics
  • Instructors / Speakers

    Course instructors
    Oliver Billker Wellcome Trust Sanger
    Institute, Hinxton, UK
    Ellen Bushell Wellcome Trust Sanger
    Institute, Hinxton, UK
    Marcus
    Lee
    Wellcome Trust Sanger Institute, Hinxton, UK
    Julian Rayner Wellcome Trust Sanger
    Institute, Hinxton, UK

    Guest speakers
    Amy Kristine Bei Laboratory of Parasitology,
    & Mycology, Aristide Le Dantec Hospital, Cheikh Anta Diop University
    of Dakar, Senegal
    Alfred Amambua-Ngwa Centre for Genomics and
    Global Health, Medical Research Council Unit, The Gambia

  • How to Apply

    Prerequisites
    The course is open to scientists and clinicians based in Africa who are
    looking to establish and use Plasmodium
    transfection technology
    at their institutes. Places are limited and
    priority will be given to
    applicants who provide a strong scientific case for
    learning
    Plasmodium genome modification. The course will be taught in
    English.

    Cost
    Overseas courses are free to attend for non-commercial
    applicants. Please contact us for the commercial fee.

    Applications
    Applications for this course can be completed online. If you have any
    problems with the online application process, please contact us.

    Please note: Applications
    must be supported by a
    recommendation from a scientific or clinical sponsor (e.g. supervisor or
    head of department). A request for a supporting
    statement will be sent
    to your nominated sponsor automatically during
    the application process.
    Applicants must ensure that their sponsor
    provides this supporting
    statement by the application deadline. Applications without a supporting
    statement cannot be considered.

    Deadlines
    Deadline for Applications: Closed

  • Cost / Bursaries

    Cost
    Overseas courses are free to attend for non-commercial applicants. Please
    contact us for the commercial fee.

    Bursaries
    Limited bursaries to cover travel, accommodation and
    sustenance costs are also available and are awarded on merit. If you
    would like to apply for a
    bursary, please complete the bursary section
    of the online application
    form.

    Please note that both the applicant
    and sponsor are required to provide
    a justification for the
    bursary as part of the application

    Bursary terms and conditions

    UK Courses (held at the Wellcome Genome Campus, Hinxton,
    Cambridge)
    A
    limited number of bursaries are available for each course. These are
    awarded by the selection committee according to merit. The bursary
    covers a maximum of 50% of the course fee, though in exceptional
    circumstances an application for the total course fee may be considered.
    Where there are many bursary applications, the selection committee may
    issue smaller amounts. We cannot assist with travel costs to attend UK
    courses.

    Overseas Courses (held outside of the UK)
    A
    limited number of bursaries are available for each course. These are
    awarded on merit to cover travel, accommodation and sustenance. The
    maximum award for travel (economy class) will be £750.

    Bursaries can be applied for as part of the course application form.
    Applicants
    will be notified of a bursary award along with their place on
    the
    course, usually within one month of the application deadline. The
    decision of the selection committee is
    final.