Malaria Experimental Genetics (Bangkok, Thailand)

22 - 27 April 2018Bangkok, Thailand
Deadlines (at 23:59 UTC):
  • Deadline for Applications Closed
  • Application Deadline Closed

  • Summary

    This free-to-attend, laboratory-based course will provide participants
    with a working knowledge of, and practical experience in, cutting-edge
    Plasmodium experimental genetics techniques; from designing gene
    targeting vectors and creating transgenic parasites to genotyping and
    phenotyping the resulting strains.

    The 2018 course will be held in Bangkok, Thalland in collaboration with
    Mahidol University Faculty of Tropical Medicine and is open to applicants
    from Asia and Australasia.

    The goal is to facilitate the participants’ own research careers by
    exposing them to state-of-the-art experimental approaches, while
    discussing the advantages and limitations of each approach. The course is
    targeted to early career malaria scientists from Asia and Australasia who
    are looking to establish and use Plasmodium transfection
    technology at their host institutes.

    The week-long course will cover experimental approaches to generating
    genetic mutations in the human malaria parasites Plasmodium
    falciparum
    and Plasmodium knowlesi The course is hands-on
    and attendees will generate gene targeting constructs, perform different
    transfection approaches, undertake practical exercises in genotyping of
    modified parasites, and both observe and learn advanced molecular
    phenotyping approaches.

    There will be a particular focus on new scalable approaches, including
    CRISPR-Cas9 genome editing. Experimental genetic approaches in other
    malaria parasites including the rodent malaria model Plasmodium
    berghei
    will also be discussed. The laboratory-based programme will
    be complemented by data analysis sessions and specific lectures in
    cutting-edge areas presented by distinguished guest speakers.

    The overall programme strategy will be to follow a Plasmodium
    experimental genetics experiment from conception to completion, equipping
    attendees to design and carry out their own projects in the future.

  • Programme

    Topics to be covered will include:

    Gene targeting vector construction

    • CRISPR-Cas9
      based approaches.
    • Consideration of the relative merits of
      different conditional expression approaches

    Transfection
    and genotyping

    • Mechanics of transfection in P.
      falciparum
      and P. knowlesi.
    • Verification of gene
      targeting using multiple genotyping techniques

    Molecular
    phenotyping

    • Imaging approaches.
    • Application of
      next generation sequencing to transcriptional profiling and quantitating
      parasite growth.
    • Flow cytometry-based phenotyping
      platforms

    Learning outcomes
    After attending this course, participants will be able to:

    • design CRISPR-Cas9 gene targeting constructs to engineer single point mutations and gene disruptions.
    • plan and execute P. falciparum and P. knowlesi transfection experiments, and genotype the resulting transfection progeny
    • evaluate the strengths and limitations of different reverse genetic techniques, including conditional gene technologies.
    • appreciate the range of phenotypic assays available for malaria experimental genetics
  • Instructors / Speakers

    Course instructors
    John Adams University of South Florida,
    USA
    Oliver Billker Wellcome Trust Sanger
    Institute, Hinxton, UK
    Ellen Bushell Wellcome Trust Sanger
    Institute, Hinxton, UK
    Tania de Koning-Ward, Deakin University,
    Australia
    Marcus Lee Wellcome Trust Sanger Institute,
    Hinxton, UK

    Guest speakers
    Thanat Chookajorn
    Faculty of Tropical Medicine, Mahidol University, Thailand
    Kesinee Chotivanich Faculty of Tropical Medicine, Mahidol University,
    Thailand
    Olivo Miotto Mahidol Oxford Tropical Medicine Research Unit
    (MORU), Thailand
    Jetsumon Prachumsri Faculty of Tropical Medicine, Mahidol University,
    Thailand
    Philip Shaw National Center for Genetic Engineering and
    Biotechnology (BIOTEC), Thailand

  • How to Apply

    Prerequisites
    The course is open to scientists and clinicians based in Asia and
    Australasia who are looking to establish and use Plasmodium
    transfection technology at their institutes. Places are limited and
    priority will be given to applicants who provide a strong scientific case
    for learning Plasmodium genome modification.

    The course will be taught in English.

    Applications
    Applications can be submitted online. Places are
    limited and will be awarded on merit. If you have any problems with the
    online application process, please contact us.

    Please note: Applications
    must be supported by a
    recommendation from a scientific or clinical sponsor (e.g. supervisor or
    head of department). A request for a supporting
    statement will be sent
    to your nominated sponsor automatically during
    the application process.
    Applicants must ensure that their sponsor
    provides this supporting
    statement by the application deadline. Applications without a
    supporting statement cannot be considered.

    Deadlines
    Deadline for Applications: Closed

  • Cost / Bursaries

    Cost
    The course is subsidised by the Wellcome Genome Campus Advanced Courses
    and Scientific Conferences Programme and is free to
    attend
    for non-commercial applicants. Please contact us for
    the commercial fee.

    Bursaries
    Limited bursaries to cover travel, accommodation and sustenance costs are
    also available and are awarded on merit. If you would like to apply for a
    bursary, please complete the bursary section of the online application
    form.

    Please note that both the applicant
    and sponsor are required to provide
    a justification for the
    bursary as part of the application

    Bursary terms and conditions

    Overseas Courses (held outside of the UK)
    A
    limited number of bursaries are available for each course. These are
    awarded on merit to cover travel, accommodation and sustenance costs. The
    maximum award for travel (economy class) is £750.

    Bursaries can be applied for as part of the course application form.
    Applicants
    will be notified of a bursary award along with their place on
    the
    course, usually within one month of the application deadline. The
    decision of the selection committee is
    final.