This course type is laboratory

Protein Interactions and Networks

09 - 17 October 2017Wellcome Genome Campus, Hinxton, UK
Deadlines (at 23:59 UTC):
  • Application Deadline Closed

  • Summary

    This popular course provides training in the latest laboratory techniques
    and bioinformatics tools necessary to discover protein interactions and
    understand protein function.

    In addition to laboratory and computational training in individual
    techniques, the course provides the opportunity to discuss how these
    methods can be integrated/applied to complementary approaches and
    strategies for the study of protein interactions and networks.

    Along with expert instructors, the practical programme will include
    lectures in topical areas as well as a panel of distinguished guest
    speakers, who will present the latest research in the field.

    Learning outcomes
    After attending the course, participants should be able to:

    • Evaluate and apply the principles and practices of different analytical methods for studying protein interactions and networks
    • Identify the strengths, weaknesses and pitfalls of each method
    • Assess how complementary analytical techniques and approaches can be integrated to answer biological questions
    • Identify suitable methods for their own research questions

    Feedback from previous courses

    • It was a great pleasure to be here. This represents one of the most significant experience as a PhD student. I hope to come back again. Muchas gracias por todo!
    • I am grateful for giving me such valuable opportunity to learn state-of-art techniques from experts in the field. I would like to contribute to the community by successfully applying the knowledge that I learned here.
    • I was very happy to attend this course. the course planning, organization and execution was excellant and was mentored nicely. the information and sceitific knowledge gained by me in this course will be of immediate use to me and fasten my research. I am also grateful for bursaries that enabled me to attend this course.
    • Thank you all for this wonderful experience!
    • Thank you very much for the very well organized, interesting and very instructive course, it was a pleasure to attend!
    • Thank you all for a truly interesting week, I’ve returned to the lab and already feel more confident and independent. I was probably the least experienced person on the course, and the intensive lab work has benefitted me massively!
  • Programme

    The programme will include lecture and practical
    laboratory/computer-based sessions covering the following topics:

    Protein Complexes by Mass Spectrometry

    • affinity purification using TAP tag approach
    • protein identification by Mass Spectrometry

    Yeast Two-Hybrid

    • rapid methods for making Bait and Prey clones
    • using mating assays to investigate specific interactions
    • library screening: faster ways to find new partners

    Binary Protein Interactions/BIAcore

    • low affinity extracellular receptor-ligand interactions by AVEXIS
    • biophysical protein interaction parameters (binding constants and kinetics)

    Protein Interactions by FRET microscopy

    • sample preparation
    • intensity-based FRET microscopy
    • Fluorescence Lifetime Imaging (FLIM)-based FRET microscopy
    • image/data analysis (e.g. ImageJ / Fiji, KNIME)

    Bioinformatics

    • network and pathway visualisation (e.g. Cytoscape)
    • protein interaction prediction and analysis
    • data integration

    Learning outcomes
    After attending the course, participants should be able to:

    • Evaluate and apply the principles and practices of different analytical methods for studying protein interactions and networks
    • Identify the strengths, weaknesses and pitfalls of each method
    • Assess how complementary analytical techniques and approaches can be integrated to answer biological questions
    • Identify suitable methods for their own research questions
  • Instructors / Speakers

    Course instructors
    Jyoti Choudhary Wellcome Trust Sanger
    Institute, Hinxton, UK
    Chris Sanderson University of Liverpool, UK
    Gavin Wright Wellcome Trust Sanger Institute, Hinxton, UK
    Anton Enright
    European Bioinformatics Institute, Hinxton, UK
    Fred S. Wouters
    University of Göttingen, Germany

    Guest speakers
    Erich Wanker Max Delbrück Center for Molecular Medicine,
    Germany
    Nadia Martinez-Martin Genentech, USA
    Edward L.
    Huttlin

    Harvard Medical School, USA
    Anne-Claude Gavin EMBL, Germany

  • How to Apply

    Prerequisites
    Applicants should be PhD students, postdoctoral scientists or
    clinicians/healthcare professionals engaged in or soon to commence
    relevant research.

    Cost
    The
    course is subsidised by the Wellcome Genome Campus Advanced Courses
    and Scientific Conferences Programme. This is a residential
    course and
    there is a fee of £1040 towards board and lodging for
    non-commercial applicants. Please contact us for the commercial fee.

    Additional limited bursaries are available (up to 50%
    of the course fee)
    and are awarded on merit. Please see the “Bursaries”
    tab for details.

    Applications
    Applications for this course can be completed online. Places are limited
    and will be awarded on merit. If you have any problems with the online
    application process, please contact us.

    Please note: Applications
    must be supported by a
    recommendation from a scientific or clinical sponsor (e.g. supervisor or
    head of department). A request for a supporting
    statement will be sent
    to your nominated sponsor automatically during
    the application process.
    Applicants must ensure that their sponsor
    provides this supporting
    statement by the application deadline. Applications without a supporting
    statement cannot be considered.

    Deadlines
    Deadline for Applications: Closed

    Travel visas
    Please contact the
    event organiser if you require a letter to support a travel visa
    application. Note that letters will be provided to confirmed attendees.

    Non-European Economic Area or Swiss nationals may be required to have a
    visa to enter the UK.
    Early application is strongly advised, as this process can take 6-8 weeks
    or longer.

    Please visit the following websites for further information:
    UK Border Agency website and information for general visitors and business
    visitors.

  • Cost / Bursaries

    Cost
    The
    course is subsidised by the Wellcome Genome Campus Advanced Courses
    and
    Scientific Conferences Programme. This is a residential
    course and
    there is a fee of £1040 towards board and lodging for
    non-commercial applicants. Please contact us for the commercial fee.

    Bursaries
    Advanced Courses are subsidised for non-commercial applicants from
    anywhere in the world. Additional, limited bursaries are
    available (up
    to 50% of the course fee) and are awarded on merit. If you would like to
    apply for a
    bursary, please complete the bursary section of the online
    application
    form.

    Please note that both the applicant
    and sponsor are required to provide
    a justification for the
    bursary as part of the application.

    Bursary terms and conditions

    UK Courses (held at the Wellcome Genome Campus, Hinxton,
    Cambridge)
    A
    limited number of bursaries are available for each course. These are
    awarded by the selection committee according to merit. The bursary
    covers a maximum of 50% of the course fee, though in exceptional
    circumstances an application for the total course fee may be considered.
    Where there are many bursary applications, the selection committee may
    issue smaller amounts. We cannot assist with travel costs to attend UK
    courses.

    Overseas Courses (held outside of the UK)
    A
    limited number of bursaries are available for each course. These are
    awarded on merit to cover travel, accommodation and sustenance. The
    maximum award for travel (economy class) will be £750.

    Bursaries can be applied for as part of the course application form.
    Applicants
    will be notified of a bursary award along with their place on
    the
    course, usually within one month of the application deadline. The
    decision of the selection committee is
    final.